Difference between revisions of "Part:BBa K3692004"

Revision as of 21:09, 23 October 2020

SAG1

Usage and Biology

Sag1 is one of the cell wall anchored proteins that contain the GPI-anchoring domain.

The presence of Sag1 inside the cell wall was indicated by the fusion of the Sag1-anchoring domain with eGFP (Inokuma et al., 2020).

It has been shown that the anchoring domain from different GPI cell wall proteins exhibit different efficiencies for the cell-surface display of target enzymes (Andreu & del Olmo, 2018)

It was observed by our team that induced expression of bacterial glucanases has the potential to cause lysis in Sacharomyces cerevisiae, (Team:Tartu TUIT - 2019.Igem.Org, n.d.). To boost the efficiency of autolysis, the localization of glucanases can be improved by fusing them with GPI-CWPs (Inokuma et al., 2020).

References

• Inokuma, K., Kurono, H., den Haan, R., van Zyl, W. H., Hasunuma, T., & Kondo, A. (2020). Novel strategy for anchorage position control of GPI-attached proteins in the yeast cell wall using different GPI-anchoring domains. Metabolic Engineering, 57, 110–117. https://doi.org/10.1016/j.ymben.2019.11.004
• Andreu, C., & del Olmo, M. (2018). Yeast arming systems: pros and cons of different protein anchors and other elements required for display. In Applied Microbiology and Biotechnology (Vol. 102, Issue 6, pp. 2543–2561). Springer Verlag. https://doi.org/10.1007/s00253-018-8827-6
• Team:Tartu TUIT - 2019.igem.org. (n.d.). Retrieved October 16, 2020, from https://2019.igem.org/Team:Tartu_TUIT

Sequence and Features