Difference between revisions of "Part:BBa K3360003"
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We chose the IARI-SP-2 endoglucanase, an endo-beta-1,4-glucanase from Bacillus subtilis. We loaded it into pGEX-4T-1-H vector as shown below. Under the induction of IPTG, IARI-SP-2 will be expressed in large quantities. For IARI-SP-2, we also have carried out codon optimization to optimize its expression in E.coli. with the GST tag. | We chose the IARI-SP-2 endoglucanase, an endo-beta-1,4-glucanase from Bacillus subtilis. We loaded it into pGEX-4T-1-H vector as shown below. Under the induction of IPTG, IARI-SP-2 will be expressed in large quantities. For IARI-SP-2, we also have carried out codon optimization to optimize its expression in E.coli. with the GST tag. | ||
| − | [[File:IARI-SP-2 Plasmid vector diagram.png|200px|center]] | + | [[File:IARI-SP-2 Plasmid vector diagram.png|200px|center]]Fig1. Plasmid vector diagram |
Revision as of 20:55, 23 October 2020
IARI-SP-2
Encoding the endo-beta-1,4-glucanase of Bacillus subtilis, the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1587
- 1000COMPATIBLE WITH RFC[1000]
SZU-China 2020 TEAM
Background
The traditional fraying process is very harmful to the environment, resources and users. In essence, being old is the destruction of cellulose on the surface of jeans. We finally consider using cellulase to achieve this effect. Through cellulase washing, it can hydrolyze the cellulose and make a part of the dye fall off the fabric. Through this effect, the "worn feeling" effect that people want can be obtained.
We chose the IARI-SP-2 endoglucanase, an endo-beta-1,4-glucanase from Bacillus subtilis. We loaded it into pGEX-4T-1-H vector as shown below. Under the induction of IPTG, IARI-SP-2 will be expressed in large quantities. For IARI-SP-2, we also have carried out codon optimization to optimize its expression in E.coli. with the GST tag.
