Difference between revisions of "Part:BBa K3450012"
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| − | + | This composite part allows E. coli to express the E2-IM2 toxin-antitoxin system sensitive to a phosphate-responsive repressor. | |
| + | This kill switch makes use of the E2-IM2 toxin-antitoxin system to make the chassis’ survival contingent on the activity of the phosphate-starvation-inducible promoter PPhoA. This dependence is established using the bacteriophage repressor cI.Under conditions of phosphate starvation, cI is expressed, shutting down the expression of the antitoxin. | ||
Revision as of 20:48, 23 October 2020
This composite part allows E. coli to express the E2-IM2 toxin-antitoxin system sensitive to a phosphate-responsive repressor. This kill switch makes use of the E2-IM2 toxin-antitoxin system to make the chassis’ survival contingent on the activity of the phosphate-starvation-inducible promoter PPhoA. This dependence is established using the bacteriophage repressor cI.Under conditions of phosphate starvation, cI is expressed, shutting down the expression of the antitoxin.