Difference between revisions of "Part:BBa K3692004"

 
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<partinfo>BBa_K3692004 short</partinfo>
 
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Alpha-agglutinin of alpha cells, C-terminal half is highly glycosylated and contains GPI anchor.
 
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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Sag1 is one of the cell wall anchored proteins that contain the GPI-anchoring domain.
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The presence of Sag1 inside the cell wall was indicated by the fusion of the Sag1-anchoring domain with eGFP (Inokuma et al., 2020).
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It has been shown that the anchoring domain from different GPI cell wall proteins exhibit different efficiencies for the cell-surface display of target enzymes (Andreu & del Olmo, 2018)
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It was observed by our team that induced expression of bacterial glucanases has the potential to cause lysis in ''Sacharomyces cerevisiae,, (''Team:Tartu TUIT - 2019.Igem.Org'', n.d.). To boost the efficiency of autolysis, the localization of glucanases can be improved by fusing them with GPI-CWPs (Inokuma et al., 2020).
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==References ==
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*Inokuma, K., Kurono, H., den Haan, R., van Zyl, W. H., Hasunuma, T., & Kondo, A. (2020). Novel strategy for anchorage position control of GPI-attached proteins in the yeast cell wall using different GPI-anchoring domains. Metabolic Engineering, 57, 110–117. https://doi.org/10.1016/j.ymben.2019.11.004
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*Andreu, C., & del Olmo, M. (2018). Yeast arming systems: pros and cons of different protein anchors and other elements required for display. In Applied Microbiology and Biotechnology (Vol. 102, Issue 6, pp. 2543–2561). Springer Verlag. https://doi.org/10.1007/s00253-018-8827-6
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*''Team:Tartu TUIT - 2019.igem.org.'' (n.d.). Retrieved October 16, 2020, from https://2019.igem.org/Team:Tartu_TUIT
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:40, 23 October 2020


SAG1


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 73
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 55
    Illegal PstI site found at 540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 493