Difference between revisions of "Part:BBa K3692002"

 
Line 3: Line 3:
 
<partinfo>BBa_K3692002 short</partinfo>
 
<partinfo>BBa_K3692002 short</partinfo>
  
−
Covers phospholipid monolayer of LDs.
 
  
−
<!-- Add more about the biology of this part here
+
''PLIN3'' is a gene that encodes perilipin - a protein which is involved both in generation of lipid droplets (LDs) and as well as covering of phospholipid monolayer of  LDs.
−
===Usage and Biology===
+
  
 +
''PLIN3'' is a human perilipin gene. However, it has been shown that the expression of this gene can increase yields of TAG by 28% in ''Sacharomyces cerevisiae'' (Teixeira et al., 2018). A potential reason for this can be that it stabilizes TAGs by improving LD assembly and the budding process.
 +
 +
== References ==
 +
*Teixeira, P. G., David, F., Siewers, V., & Nielsen, J. (2018). Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae. FEMS Yeast Research, 18(6). https://doi.org/10.1093/femsyr/foy060
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:26, 23 October 2020


PLIN3


PLIN3 is a gene that encodes perilipin - a protein which is involved both in generation of lipid droplets (LDs) and as well as covering of phospholipid monolayer of LDs.

PLIN3 is a human perilipin gene. However, it has been shown that the expression of this gene can increase yields of TAG by 28% in Sacharomyces cerevisiae (Teixeira et al., 2018). A potential reason for this can be that it stabilizes TAGs by improving LD assembly and the budding process.

References

  • Teixeira, P. G., David, F., Siewers, V., & Nielsen, J. (2018). Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae. FEMS Yeast Research, 18(6). https://doi.org/10.1093/femsyr/foy060

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 369
    Illegal PstI site found at 218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 369
    Illegal PstI site found at 218
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 369
    Illegal PstI site found at 218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 369
    Illegal PstI site found at 218
  • 1000
    COMPATIBLE WITH RFC[1000]