Difference between revisions of "Part:BBa K3582004:Design"

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(References)
 
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===References===
 
===References===
*[1] Baratin, M., Roetynck, S., Pouvelle, B., Lemmers, C., Viebig, N. K., Johansson, S., . . . Ugolini, S. (2007, February 21). Dissection of the role of PfEMP1 and ICAM-1 in the sensing of Plasmodium-falciparum-infected erythrocytes by natural killer cells. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1794133/
 
*[2]Hviid L, Jensen AT. PfEMP1 ‐ A parasite protein family of key importance in Plasmodium falciparum malaria immunity and pathogenesis. Adv Parasitol. 2015;88:51‐84
 
*[3]Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA. Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell. 1988;52:925–933.
 
*[4]A. Bengtsson et a., A novel domain cassette identifies Plasmodium falciparum PfEMP1 proteins binding ICAM-1 and is a target of cross-reactive, adhesion-inhibitory antibodies. J. Immunol. 190, 240–249 (2013).
 
*[5]E. Carrington et al., In silico guided reconstruction and analysis of ICAM-1-binding var genes from Plasmodium falciparum. Sci. Rep. 8, 3282 (2018)
 
*[6] D. P. G. Howell et al., Mapping a common interaction site used by Plasmodium falciparum Duffy binding-like domains to bind diverse host receptors. Mol. Microbiol. 67, 78–87 (2008).CrossRefPubMedGoogle Scholar
 
*[7] H. Janes et al., Investigating the host binding signature on the Plasmodium falciparum PfEMP1 protein family. PLoS Pathog. 7, e1002032 (2011).
 

Latest revision as of 18:09, 23 October 2020


The DBLb domain of PF11_0521 PfEMP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1113
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • 1] RFC10 criteria satisfied
  • 2] 6x-His-tag gene added for purification
  • 3] Codon optimized with E-coli K-12 strain

Source

Protein sequence was obtained from PDB and reverse translated to give this biobrick

References