Difference between revisions of "Part:BBa K256004"
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==MIT MAHE 2020==
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==MIT_MAHE 2020==
 
'''Summary'''
 
'''Summary'''
 
<p>Nitric oxide (NO) induces NO-detoxifying enzymes in Escherichia coli suggesting sensitive mechanisms for coordinate control of NO defense genes in response to NO stress. Exposure of E. coli to sub-micromolar NO levels under anaerobic conditions rapidly induces transcription of the NO reductase (NOR) structural genes. This protein is involved in the pathway nitric oxide reduction, which is part of Nitrogen metabolism. It also acts as a transcriptional activator for at least the norVW operon. It binds to the promoter region of norVW, to a consensus target sequence, GT-(N7)-AC, which is highly conserved among proteobacteria.</p>
 
<p>Nitric oxide (NO) induces NO-detoxifying enzymes in Escherichia coli suggesting sensitive mechanisms for coordinate control of NO defense genes in response to NO stress. Exposure of E. coli to sub-micromolar NO levels under anaerobic conditions rapidly induces transcription of the NO reductase (NOR) structural genes. This protein is involved in the pathway nitric oxide reduction, which is part of Nitrogen metabolism. It also acts as a transcriptional activator for at least the norVW operon. It binds to the promoter region of norVW, to a consensus target sequence, GT-(N7)-AC, which is highly conserved among proteobacteria.</p>

Latest revision as of 17:39, 23 October 2020


NorR

DNA-binding transcriptional activator, B2709, NorR, YgaA, transcriptional regulator of the anaerobic response to reactive nitrogen species

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430


MIT_MAHE 2020

Summary

Nitric oxide (NO) induces NO-detoxifying enzymes in Escherichia coli suggesting sensitive mechanisms for coordinate control of NO defense genes in response to NO stress. Exposure of E. coli to sub-micromolar NO levels under anaerobic conditions rapidly induces transcription of the NO reductase (NOR) structural genes. This protein is involved in the pathway nitric oxide reduction, which is part of Nitrogen metabolism. It also acts as a transcriptional activator for at least the norVW operon. It binds to the promoter region of norVW, to a consensus target sequence, GT-(N7)-AC, which is highly conserved among proteobacteria.

References

1. Gardner, A. M., Gessner, C. R., & Gardner, P. R. (2003). Regulation of the nitric oxide reduction operon (norRVW) in Escherichia coli. Role of NorR and sigma54 in the nitric oxide stress response. The Journal of biological chemistry, 278(12), 10081–10086. https://doi.org/10.1074/jbc.M212462200

2. Roselle, D. C., & Smith, D. J. (1998). Characterization and nitric oxide release studies of lipophilic 1-substituted diazen-1-ium-1,2-diolates. Journal of controlled release : official journal of the Controlled Release Society, 51(2-3), 131–142. https://doi.org/10.1016/s0168-3659(97)00148-x