Difference between revisions of "Part:BBa K3482007"

Revision as of 16:26, 23 October 2020

8x Histidine tag for protein purification

This 8x Histidine tag can be used for the affinity purification of a tagged protein.

The His tag has been used to purify the azurin secreted outside of the E.coli Nissle 1917 cells.

Sequence and Features

Assembly Compatibility:

Revision as of 16:23, 23 October 2020 (view source) Santosmoreno j (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 16:26, 23 October 2020 (view source) Santosmoreno j (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K3482007 short</partinfo>		<partinfo>BBa_K3482007 short</partinfo>

−	This 8x Histidine tag can be used ~~to purify~~ a protein~~. The tag has to be fused with his protein to be used in a purification~~.	+	This 8x Histidine tag can be used for the affinity purification of a tagged protein.