Difference between revisions of "Part:BBa K117000:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Lysis gene was commercially synthesized by ****.  
+
Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke]
 
==Extraction of Lysis gene/PCR Oligo fragments DNA sequence
 
==Extraction of Lysis gene/PCR Oligo fragments DNA sequence
 
Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.
 
Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.

Revision as of 13:34, 29 October 2008

Lysis gene (promotes lysis in colicin-producing bacteria strain)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke] ==Extraction of Lysis gene/PCR Oligo fragments DNA sequence Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.

Source of Orgin sequence

to be updated.

Source

to be updated

References