Difference between revisions of "Part:BBa K3561030"

Latest revision as of 15:51, 23 October 2020

WWTQNKY-CBD

This fusion is a fusion of our palladium reducing peptide and cellulose binding domain. The palladium reducing peptide is expected to reduce palladium and the cellulose binding domain is expected to adsorb to cellulose. More details of the palladium reducing peptide can be found in part BBa_K3561025, this is one of the best performing peptides. The sequence of the cellulose binding domain is provided by Prod. Shen Long Tsai and can be found in part BBa_K3561026.

We have added a NheI and a PStI cut site at the start and end of the sequence respectively. An EcoRI cut site is added in between the peptide and the CBD. The codons are also optimised.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 22
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 22
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 22
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 22
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 22
Illegal AgeI site found at 130
Illegal AgeI site found at 340
1000
COMPATIBLE WITH RFC[1000]

References

Yunus, Ian Sofian, and Shen-Long Tsai. “Designed Biomolecule–Cellulose Complexes for Palladium Recovery and Detoxification.” RSC Advances, The Royal Society of Chemistry, 12 Feb. 2015, pubs.rsc.org/en/content/articlelanding/2015/ra/c4ra16200e.

@@ Line 14: / Line 14: @@
 <partinfo>BBa_K3561030 SequenceAndFeatures</partinfo>
+<h2>References</h2>
+Yunus, Ian Sofian, and Shen-Long Tsai. “Designed Biomolecule–Cellulose Complexes for Palladium Recovery and Detoxification.” RSC Advances, The Royal Society of Chemistry, 12 Feb. 2015, pubs.rsc.org/en/content/articlelanding/2015/ra/c4ra16200e.
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