Difference between revisions of "Part:BBa K3468030:Design"

 
 
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===Design Notes===
 
===Design Notes===
NONE
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By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. Y219W was designed by searching for suitable sites around F229 for mutation.
 
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===Source===
 
===Source===
  

Latest revision as of 13:31, 23 October 2020


PETase Y219W


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. Y219W was designed by searching for suitable sites around F229 for mutation.

Source

Ideonalla sakaiensis

References