Difference between revisions of "Part:BBa K3468020:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around L249 and I250 for mutations, the hydrophobic interaction force between the residues was enhanced and the hydrophobic core was created, so N246I was designed. | |
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===Source=== | ===Source=== | ||
Latest revision as of 13:18, 23 October 2020
PETase N246I
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around L249 and I250 for mutations, the hydrophobic interaction force between the residues was enhanced and the hydrophobic core was created, so N246I was designed.
Source
Ideonalla sakaiensis