Difference between revisions of "Part:BBa K3510007"
Lealinevogt (Talk | contribs) |
|||
| Line 3: | Line 3: | ||
<partinfo>BBa_K3510007 short</partinfo> | <partinfo>BBa_K3510007 short</partinfo> | ||
| − | to | + | <b><font color= red>Important: This construct does not include a RBS upstream of the mRFP sequence. When building on our design please make sure you add it in order to allow for translation</font color= red></b> |
| + | |||
| + | This composite part is a Vitamin B12 inducible expression system of a mRFP (BBa_E1010). The included part BBa_K1913008 contains a constitutive Promoter (BBa_J23100) and a riboswitch. Upon binding of Vitamin B12, the riboswitch prevents translation of the following gene. To express mRFP in the presence of Vitamin B12, a Tet inverter system (BBa_Q04400) is placed in between. When the encoded repressor is not translated anymore, the mRFP is expressed again. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015). | ||
| + | This part should have replicated the biobrick BBa_K1913011 by Team Wageningen 2016. | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 12:18, 23 October 2020
Anderson-Promoter-B12-Riboswitch-Tet-Inverter-System-mRFP-Terminator
Important: This construct does not include a RBS upstream of the mRFP sequence. When building on our design please make sure you add it in order to allow for translation
This composite part is a Vitamin B12 inducible expression system of a mRFP (BBa_E1010). The included part BBa_K1913008 contains a constitutive Promoter (BBa_J23100) and a riboswitch. Upon binding of Vitamin B12, the riboswitch prevents translation of the following gene. To express mRFP in the presence of Vitamin B12, a Tet inverter system (BBa_Q04400) is placed in between. When the encoded repressor is not translated anymore, the mRFP is expressed again. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015). This part should have replicated the biobrick BBa_K1913011 by Team Wageningen 2016.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 199
Illegal AgeI site found at 1929
Illegal AgeI site found at 2041 - 1000COMPATIBLE WITH RFC[1000]