Difference between revisions of "Part:BBa K3596028"

 
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BIOT is a basic design for the expression of <i>Geobacter sulfurreducens</i>' pilin-based electrically conductive protein nanowires (e-PNs)<sup>[1]</sup>. The system includes a tac promoter which promotes the pilin gene(<i>pilA</i>) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.</p>
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<img src="https://2020.igem.org/wiki/images/6/64/T--GreatBay_SZ--circuit2.png" alt="" width="700">
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<h6 style="text-align:center">Figure 1:  <strong>A</strong> Construction of BIOT gene circuit</h6> 
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<p>In order to improve the protein nanowire, we mutated the <i>pilA</i> gene to enhance the productivity and output voltage. <b>This part codes for mutated pilin gene with four more carboxylic groups in each protein monomer(Q55E)</b>.The electricity productivity of this part is shown in figure 2.
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<img src="https://2020.igem.org/wiki/images/7/75/T--GreatBay_SZ--protein_mutant.png" alt="" width="700">
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<h6 style="text-align:center">Figure 2:  Electricity productivity of engineered BIOT system with different pilin mutant. </h6> 
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<p><b>References</b></p>
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<p>[1] Liu X, Gao H, Ward J E, <i>et al</i>. Power generation from ambient humidity using protein nanowires[J]. <i>Nature</i>, 2020, 578(7796): 550-554.</p>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3596028 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3596028 parameters</partinfo>
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Revision as of 11:54, 23 October 2020

Pilin Gene mut 4D

BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)[1]. The system includes a tac promoter which promotes the pilin gene(pilA) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.

Figure 1: A Construction of BIOT gene circuit

In order to improve the protein nanowire, we mutated the pilA gene to enhance the productivity and output voltage. This part codes for mutated pilin gene with four more carboxylic groups in each protein monomer(Q55E).The electricity productivity of this part is shown in figure 2.

Figure 2: Electricity productivity of engineered BIOT system with different pilin mutant.

References

[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]