Difference between revisions of "Part:BBa K3596007"

 
 
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__NOTOC__
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<partinfo>BBa_K3596007 short</partinfo>
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<p>
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BIOT is a basic design for the expression of <i>Geobacter sulfurreducens</i>' pilin-based electrically conductive protein nanowires (e-PNs)<sup>[1]</sup>. The system includes a tac promoter which promotes the pilin gene(<i>pilA</i>) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.</p>
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This part codes for the assembly machinery of <i>Geobacter sulfurreducens</i>' pilin-based electrically conductive protein nanowires(e-PNs), a part of the BIOT system
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<div style="text-align: center;">
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<img src="https://2020.igem.org/wiki/images/6/64/T--GreatBay_SZ--circuit2.png" alt="" width="700">
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<h6 style="text-align:center">Figure 1:  <strong>A</strong> Construction of BIOT gene circuit</h6> 
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<p><b>References</b></p>
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<p>[1] Liu X, Gao H, Ward J E, <i>et al</i>. Power generation from ambient humidity using protein nanowires[J]. <i>Nature</i>, 2020, 578(7796): 550-554.</p>
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</html>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3596007 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3596007 parameters</partinfo>
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Latest revision as of 11:04, 23 October 2020

ppdA+ppdB+ygdB+ppdC+gspO

BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)[1]. The system includes a tac promoter which promotes the pilin gene(pilA) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.

This part codes for the assembly machinery of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires(e-PNs), a part of the BIOT system
Figure 1: A Construction of BIOT gene circuit

References

[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 764
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1770