Difference between revisions of "Part:BBa K3380500:Design"

(→‎Source)
(→‎Design Notes)
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase.  
 
All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase.  
−
The method was similar to the one used by Millacura ''et al.'' 2019.
+
The method was similar to the one used by Millacura ''et al.'' 2019. (for more details check the experience page)
  
 
===Source===
 
===Source===

Revision as of 09:57, 23 October 2020


iSpinach fluorescent RNA aptamer construct under T7 RNA polymerase promoter (BBa_z0251)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


Design Notes

All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase. The method was similar to the one used by Millacura et al. 2019. (for more details check the experience page)

Source

The parts were acquired as sequences from Sigma-Aldrich

References

Millacura, F.A., Li, M., Valenzuela-Ortega, M.A. and French, C., 2019. TXO: Transcription-Only genetic circuits as a novel cell-free approach for Synthetic Biology. bioRxiv, p.826230.