Difference between revisions of "Part:BBa K3487005"
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<partinfo>BBa_K3487005 short</partinfo>
 
<partinfo>BBa_K3487005 short</partinfo>
 
===Description===
 
===Description===
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After being expressed, RalR acts as a non-specific endonuclease that can cleave methylated and unmethylated DNA, leading to cell death. We put it as a poisonous protein into the suicide switch.
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RalR acts as a non-specific endonuclease that can cleave methylated and unmethylated DNA, leading to cell death.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 07:10, 23 October 2020


Non-specific endonuclease

Description

RalR acts as a non-specific endonuclease that can cleave methylated and unmethylated DNA, leading to cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2020 SZPT-CHINA

Result

We streaked the engineered strain into a 1Mm IPTG plate for overnight culture, and observed that the engineered strain containing the RalR gene did not grow on the plate. The expression of RalR was good under the positive and negative controls, and the colony situation in the corresponding area was significantly less than that of the empty vector control group. The result is shown in the following.

T--SZPT-China--RalR.png

References

Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.