Difference between revisions of "Part:BBa K3573000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The engineering is based on the structure of RAS: anti-RAS antibody (PDB ID: 2vh5)
 
  
 +
This year's project is a continuation of 2019's project. Last year our team (Korea_HS) designed and tested CPP-scFv(P5) for its ability to enter the cell and recognize its cognate target protein, lysozyme in a reducing environment.
  
 +
In 2020, we have designed hyperstable, cell-penetrating scFv targeting RAS protein (scFv(RAS)) based on the structure of RAS: anti-RAS antibody (PDB ID: 2vh5)
  
 
===Source===
 
===Source===

Revision as of 03:53, 23 October 2020


Single chain variable fragment (scFV) recognizing RAS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This year's project is a continuation of 2019's project. Last year our team (Korea_HS) designed and tested CPP-scFv(P5) for its ability to enter the cell and recognize its cognate target protein, lysozyme in a reducing environment.

In 2020, we have designed hyperstable, cell-penetrating scFv targeting RAS protein (scFv(RAS)) based on the structure of RAS: anti-RAS antibody (PDB ID: 2vh5)

Source

This DNA was synthesized

References