Difference between revisions of "Part:BBa K3328000:Experience"
(→Applications of BBa_K3328000) |
(→Applications of BBa_K3328000) |
||
| Line 5: | Line 5: | ||
===Applications of BBa_K3328000=== | ===Applications of BBa_K3328000=== | ||
| + | ====3WJ repressor==== | ||
| + | |||
https://2020.igem.org/wiki/images/thumb/c/c6/T--OUC-China--result_fig3.jpg/653px-T--OUC-China--result_fig3.jpg | https://2020.igem.org/wiki/images/thumb/c/c6/T--OUC-China--result_fig3.jpg/653px-T--OUC-China--result_fig3.jpg | ||
| Line 14: | Line 16: | ||
3WJ repressor showed remarkable ON/OFF ratio and had good orthogonality, we choose it as OFF switch. And we used it in the construction of logic gates and the design of some more complex circuits. | 3WJ repressor showed remarkable ON/OFF ratio and had good orthogonality, we choose it as OFF switch. And we used it in the construction of logic gates and the design of some more complex circuits. | ||
| + | |||
| + | ==== NOT gate==== | ||
| + | |||
| + | https://2020.igem.org/wiki/images/thumb/4/4b/T--OUC-China--result_fig7.jpg/800px-T--OUC-China--result_fig7.jpg | ||
| + | |||
| + | The left figure shows that when INPUT=0, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low when INPUT=1. This corresponds to the situation described in the truth table on the right. INPUT=1 means that aTc (0.25 mg/ml) is added. | ||
| + | |||
| + | ==== IMPLY gate==== | ||
| + | |||
| + | https://2020.igem.org/wiki/images/thumb/5/52/T--OUC-China--part_IMPLY1.jpg/800px-T--OUC-China--part_IMPLY1.jpg | ||
| + | |||
| + | https://2020.igem.org/wiki/images/thumb/6/66/T--OUC-China--part_IMPLY2.jpg/800px-T--OUC-China--part_IMPLY2.jpg | ||
| + | |||
| + | The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 02:26, 23 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3328000
3WJ repressor
In the validation of 3WJ, as with toehold, we also set blank control (IPTG=0 M, aTc=0 mg/ml). Compared with the group without trigger expression (IPTG=0.1 M, aTc=0 mg/ml), the group with trigger expression (IPTG=0.1 M, aTc=0.25 mg/ml) showed inhibitory effect.
Crosstalk was determined by dividing the arithmetic mean of the GFP fluorescence from a given trigger switch pair by the arithmetic mean of the GFP fluorescence for the cognate trigger switch interaction. GFP fluorescence was measured from n=9 biologically independent samples.
3WJ repressor showed remarkable ON/OFF ratio and had good orthogonality, we choose it as OFF switch. And we used it in the construction of logic gates and the design of some more complex circuits.
NOT gate
The left figure shows that when INPUT=0, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low when INPUT=1. This corresponds to the situation described in the truth table on the right. INPUT=1 means that aTc (0.25 mg/ml) is added.
IMPLY gate
The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
User Reviews
UNIQ0a6a4add179b4eb9-partinfo-00000000-QINU UNIQ0a6a4add179b4eb9-partinfo-00000001-QINU