Difference between revisions of "Part:BBa K3510006"

Revision as of 21:17, 22 October 2020

Anderson-Promoter-B12-Riboswitch-Tet-Inverter-System-GFP-Terminator

This composite part is a Vitamin B12 inducible expression system of a superfolder GFP (BBa_I746916). The included part BBa_K1913008 contains a constitutive Promoter (BBa_J23100) and a riboswitch. Upon binding of Vitamin B12, the riboswitch prevents translation of the following gene. To express GFP in the presence of Vitamin B12, a Tet inverter system (BBa_Q04400) is placed in between. When the encoded repressor is not translated anymore, the GFP is expressed again. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015). This part was inspired by Team Wageningen 2016, who provided this construct with mRFP (BBa_K1913011). By changing the reporter protein to superfold GFP, we seeked to provide an additional option for future iGEM teams of which the expression can be measured reliably using the fluorescein-based iGEM fluorescence calibration protocol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 199
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1401

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3510006 short</partinfo>
-to be filled
+This composite part is a Vitamin B12 inducible expression system of a superfolder GFP (BBa_I746916). The included part BBa_K1913008 contains a constitutive Promoter (BBa_J23100) and a riboswitch. Upon binding of Vitamin B12, the riboswitch prevents translation of the following gene. To express GFP in the presence of Vitamin B12, a Tet inverter system (BBa_Q04400) is placed in between. When the encoded repressor is not translated anymore, the GFP is expressed again. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015).
+This part was inspired by Team Wageningen 2016, who provided this construct with mRFP (BBa_K1913011). By changing the reporter protein to superfold GFP, we seeked to provide an additional option for future iGEM teams of which the expression can be measured reliably using the fluorescein-based iGEM fluorescence calibration protocol.
 <!-- Add more about the biology of this part here