Difference between revisions of "Part:BBa K3510002"
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This composite part is a manganese inducible expression system of a blue chromoprotein (BBa_K864401) additionally tagged with FAST2 (BBa_K3510000). Expression control consists of two individual elements: The manganese inducible promoter (BBa_K902073) and a manganese riboswitch (BBa_K902074). Transcription is regulated by the promoter, while the riboswitch supports translation initiation only in the presence of manganese. We combined both elements hoping for an even tighter regulation. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015). | This composite part is a manganese inducible expression system of a blue chromoprotein (BBa_K864401) additionally tagged with FAST2 (BBa_K3510000). Expression control consists of two individual elements: The manganese inducible promoter (BBa_K902073) and a manganese riboswitch (BBa_K902074). Transcription is regulated by the promoter, while the riboswitch supports translation initiation only in the presence of manganese. We combined both elements hoping for an even tighter regulation. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015). | ||
| − | + | <b>Usage:</b> | |
In our project we coupled the manganese sensing device with a chromoprotein to have an easy-to-use biosensor that gives a qualitative signal by color. This should allow in field testing without the necessity of a laboratory providing a equipment. Therefore, this construct could be really beneficial to roughly examine water quality in areas with poor infrastructure. | In our project we coupled the manganese sensing device with a chromoprotein to have an easy-to-use biosensor that gives a qualitative signal by color. This should allow in field testing without the necessity of a laboratory providing a equipment. Therefore, this construct could be really beneficial to roughly examine water quality in areas with poor infrastructure. | ||
Additionally, this construct was designed as a control for the effect of phytochelatin on the sensing mechanism in the construct BBa_K3510003. Unlike the phytochelatin the chromoprotein should not interfere with manganese. | Additionally, this construct was designed as a control for the effect of phytochelatin on the sensing mechanism in the construct BBa_K3510003. Unlike the phytochelatin the chromoprotein should not interfere with manganese. | ||
Revision as of 17:04, 22 October 2020
Mn-Promoter-Mn-Riboswitch-FAST-Chromoprotein-Terminator
This composite part is a manganese inducible expression system of a blue chromoprotein (BBa_K864401) additionally tagged with FAST2 (BBa_K3510000). Expression control consists of two individual elements: The manganese inducible promoter (BBa_K902073) and a manganese riboswitch (BBa_K902074). Transcription is regulated by the promoter, while the riboswitch supports translation initiation only in the presence of manganese. We combined both elements hoping for an even tighter regulation. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015).
Usage: In our project we coupled the manganese sensing device with a chromoprotein to have an easy-to-use biosensor that gives a qualitative signal by color. This should allow in field testing without the necessity of a laboratory providing a equipment. Therefore, this construct could be really beneficial to roughly examine water quality in areas with poor infrastructure. Additionally, this construct was designed as a control for the effect of phytochelatin on the sensing mechanism in the construct BBa_K3510003. Unlike the phytochelatin the chromoprotein should not interfere with manganese.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 319