Difference between revisions of "Part:BBa K3482016"

 
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<partinfo>BBa_K3482016 short</partinfo>
  
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This part allows for IPTG inducible expression of the ccdB toxin in E. coli which lead to cell death by acting on the DNA gyrase. the action of ccdB leads to the degradation of DNA , which leads to cell death. It can therefore be induced by IPTG.
  
 
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Revision as of 14:57, 22 October 2020


IPTG-inducible ccdb toxin

This part allows for IPTG inducible expression of the ccdB toxin in E. coli which lead to cell death by acting on the DNA gyrase. the action of ccdB leads to the degradation of DNA , which leads to cell death. It can therefore be induced by IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 307