Difference between revisions of "Part:BBa K3482016"
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<partinfo>BBa_K3482016 short</partinfo> | <partinfo>BBa_K3482016 short</partinfo> | ||
− | + | This part allows for IPTG inducible expression of the ccdB toxin in E. coli which lead to cell death by acting on the DNA gyrase. the action of ccdB leads to the degradation of DNA , which leads to cell death. It can therefore be induced by IPTG. | |
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Revision as of 14:57, 22 October 2020
IPTG-inducible ccdb toxin
This part allows for IPTG inducible expression of the ccdB toxin in E. coli which lead to cell death by acting on the DNA gyrase. the action of ccdB leads to the degradation of DNA , which leads to cell death. It can therefore be induced by IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 307