Difference between revisions of "Part:BBa K3328052:Design"
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===Design Notes=== | ===Design Notes=== | ||
In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on. | In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 14:26, 22 October 2020
triggerB of NIMPLY gate (NIMPLY2)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 17
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 14
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 17
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 17
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.
Source
synthesize from company
References
[1] Green, A. A., Kim, J., Ma, D., Silver, P. A., Collins, J. J., & Yin, P. (2017). Complex cellular logic computation using ribocomputing devices. Nature, 548(7665), 117–121. doi:10.1038/nature23271
[2] Green, A. A., Silver, P. A., Collins, J. J., and Yin, P. (2014) toehold switches: de-novo-designed regulators of gene expression. Cell 159, 925– 939, DOI: 10.1016/j.cell.2014.10.002