Difference between revisions of "Part:BBa K123001"
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When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli. | When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli. | ||
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[[image:bpa.jpeg]] | [[image:bpa.jpeg]] | ||
Figure 3 BPA degradation by bisdB- and bisdAB-recombinant cells in L-BPA medium. The symbols represent the BPA content remaining in L-BPA medium in which E. coli BL21 (DE3) cells bearing pET17b (circles), pET17bisdB (triangles), and pET17bisdAB (squares) were grown. Error bars indicate standard deviations from three independent experiments. | Figure 3 BPA degradation by bisdB- and bisdAB-recombinant cells in L-BPA medium. The symbols represent the BPA content remaining in L-BPA medium in which E. coli BL21 (DE3) cells bearing pET17b (circles), pET17bisdB (triangles), and pET17bisdAB (squares) were grown. Error bars indicate standard deviations from three independent experiments. | ||
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M. Sasaki, T. Tsuchido, Y. Matsumura. 2008 Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1. Journal of Applied Microbiology 105:4:1158-1169 | M. Sasaki, T. Tsuchido, Y. Matsumura. 2008 Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1. Journal of Applied Microbiology 105:4:1158-1169 | ||
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| + | ===Usage and Biology=== | ||
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Revision as of 03:06, 29 October 2008
BisdB
When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli.
Figure 3 BPA degradation by bisdB- and bisdAB-recombinant cells in L-BPA medium. The symbols represent the BPA content remaining in L-BPA medium in which E. coli BL21 (DE3) cells bearing pET17b (circles), pET17bisdB (triangles), and pET17bisdAB (squares) were grown. Error bars indicate standard deviations from three independent experiments.
Figure Taken From:
M. Sasaki, T. Tsuchido, Y. Matsumura. 2008 Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1. Journal of Applied Microbiology 105:4:1158-1169
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 479
Illegal BamHI site found at 867 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 1276 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1086