Difference between revisions of "Part:BBa K3504017"
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==Usage== | ==Usage== | ||
| + | SP6 promoter controls the transcription of the nsPs genes of pSFV; therefore, in vitro transcription and capping of the 5′ end are required for the expression of the gene of interest in mammalian cells. | ||
| + | |||
==Characterization== | ==Characterization== | ||
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Revision as of 12:46, 22 October 2020
SP6 Promoter
Part Description
Sp6 is a constitutive promoter from Sp6 bacteriophage but requires SP6 RNA polymerase When used for in vitro transcription, and according to its orientation the promoter transcribes either the sense OR antisense transcript.
Usage
SP6 promoter controls the transcription of the nsPs genes of pSFV; therefore, in vitro transcription and capping of the 5′ end are required for the expression of the gene of interest in mammalian cells.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]