Difference between revisions of "Part:BBa K3394008"

Revision as of 12:10, 22 October 2020

Cellulolytic complex [2] with AIDA-I

Our project's goal is to decompose cellulose fibered wet wipes in the sewer systems to prevent the formation of "fatbergs" and clogs. Since cellulose cannot cross the membrane, we engineered E. coli to express cellulolytic complex on its surface through AIDA-1 auto-display system. For more information about AIDA-1 auto-display system, see BBa_K3394006.

Figure 1: decomposition of cellulose fibered wet wipes by engineered E. coli

Our cellulolytic complex is composed of:

1. Cellulase enzyme: We used Endo5a cellulase, which breaks 1,4 beta bonds. In the future, it would help us degrade wet wipes based on cellulose fibers. For more information see BBa_K3394000.

2. CBD-cellulose binding domain. Its purpose is to bind the cellulose polymer thus, enriching the cellulose surface with cellulose degrading bacteria, so the degradation will be more efficient. For more information see BBa_K3394001.

3. His-tag: For more information see BBa_K3394002.

4. GS linker: For more information see BBa_K3394003.

Figure 2: Complete cellulolytic complex expressed on the bacteria's surface by AIDA-1 auto-display system.

We have another design in which the order of the passenger genes is different (BBa_K3394007).

Sequence and Features