Difference between revisions of "Part:BBa K3394005:Design"
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Latest revision as of 12:06, 22 October 2020
Cellulolytic complex [2]: His-CBD-GS-Endo5a
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623
Illegal BamHI site found at 28 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1623
Illegal AgeI site found at 51 - 1000COMPATIBLE WITH RFC[1000]
Source
References
Adlakha, N., Rajagopal, R., Kumar, S., Reddy, V. S., & Yazdani, S. S. (2011). Synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect gut bacterium. Applied and environmental microbiology, 77(14), 4859-4866.
Gupta, S., Adlakha, N., & Yazdani, S. S. (2013). Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli. Protein expression and purification, 88(1), 20-25.