Difference between revisions of "Part:BBa K3394003:Design"

(References)
(Source)
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
Artificial sequence - synthetic construct
+
GenBank: HQ657205.1 (NCBI)
  
 
===References===
 
===References===
 
Lu, P., & Feng, M. G. (2008). Bifunctional enhancement of a β-glucanase-xylanase fusion enzyme by optimization of peptide linkers. Applied microbiology and biotechnology, 79(4), 579-587.
 
Lu, P., & Feng, M. G. (2008). Bifunctional enhancement of a β-glucanase-xylanase fusion enzyme by optimization of peptide linkers. Applied microbiology and biotechnology, 79(4), 579-587.

Latest revision as of 11:48, 22 October 2020


Glycine-serine (GS) linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to include GS linker in our design to keep the catalytic domain of endo5a separate from the cellulose-binding domain (CBD).


Source

GenBank: HQ657205.1 (NCBI)

References

Lu, P., & Feng, M. G. (2008). Bifunctional enhancement of a β-glucanase-xylanase fusion enzyme by optimization of peptide linkers. Applied microbiology and biotechnology, 79(4), 579-587.