Difference between revisions of "Part:BBa K3394001"
 
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The binding domain improves the attachment of the enzyme to the substrate and facilitate the catalytic domain activity.
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The binding domain improves the attachment of the enzyme to the substrate and facilitates the catalytic domain activity.
 
Similar binding domain structures can be found in complexes degrading other insoluble carbohydrates such as chitin.  
 
Similar binding domain structures can be found in complexes degrading other insoluble carbohydrates such as chitin.  
  
 
[[File:T--BGU-Israel--CBDimage.jpeg|300px|thumb|center|Figure 1: CBD - Cellulose-Binding Domains]]
 
[[File:T--BGU-Israel--CBDimage.jpeg|300px|thumb|center|Figure 1: CBD - Cellulose-Binding Domains]]
  
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The CBD sequence we used was cloned into an Escherichia coli expression vector (PET-28) in the lab.
 
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 11:43, 22 October 2020


Coding Sequence of CBD (Cellulose Binding Domain)

Most cellulolytic complexes consist of catalytic domains (Cellulases) and Cellulose-Binding Domains (CBDs).


The binding domain improves the attachment of the enzyme to the substrate and facilitates the catalytic domain activity. Similar binding domain structures can be found in complexes degrading other insoluble carbohydrates such as chitin.

Figure 1: CBD - Cellulose-Binding Domains


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 37
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 37
    Illegal AgeI site found at 12
  • 1000
    COMPATIBLE WITH RFC[1000]