Difference between revisions of "Part:BBa K3394001:Design"
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===Source=== | ===Source=== | ||
| − | + | GenBank: CAA48312.1 (NCBI) | |
===References=== | ===References=== | ||
Szczupak, A., Aizik, D., Moraïs, S., Vazana, Y., Barak, Y., Bayer, E. A., & Alfonta, L. (2017). The electrosome: a surface-displayed enzymatic cascade in a biofuel cell’s anode and a high-density surface-displayed biocathodic enzyme. Nanomaterials, 7(7), 153. | Szczupak, A., Aizik, D., Moraïs, S., Vazana, Y., Barak, Y., Bayer, E. A., & Alfonta, L. (2017). The electrosome: a surface-displayed enzymatic cascade in a biofuel cell’s anode and a high-density surface-displayed biocathodic enzyme. Nanomaterials, 7(7), 153. | ||
Latest revision as of 11:42, 22 October 2020
Coding Sequence of CBD (Cellulose Binding Domain)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 37
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 37
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 37
Illegal AgeI site found at 12 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The CBD sequence we used was cloned into an Escherichia coli expression vector (PET-28) in the lab.
Source
GenBank: CAA48312.1 (NCBI)
References
Szczupak, A., Aizik, D., Moraïs, S., Vazana, Y., Barak, Y., Bayer, E. A., & Alfonta, L. (2017). The electrosome: a surface-displayed enzymatic cascade in a biofuel cell’s anode and a high-density surface-displayed biocathodic enzyme. Nanomaterials, 7(7), 153.