Difference between revisions of "Part:BBa K3447102"

Revision as of 11:06, 22 October 2020

Arabinose production

P_C is a constitutive promoter that expresses the downstream araC gene. P_BAD promoter is an inducible promoter, P_BAD contains sequences of P_C. It was verified by iGEM 2017 Amazonas_Brazil team that the induction effect of arabinose could be realized by using only araC and P_BAD.

Usage and Biology

Arabinose operon is composed of AraC protein, P_BAD and P_C. The araC gene translates from the P_C to the left.
We used the P_C promoter to express AraC constitutively, and constituted the sensing part of the arabinose sensing system.
In the arabinose operon, it is regulated by the AraC operon and activates the expression of downstream arabinose metabolism genes in the presence of arabinose inducers.
The L-Arabinose operon is naturally present in E. coli.The regulatory protein AraC ACTS as a dimer. The operon contains two cis-type control elements: The PC promoter for the synthesis of AraC and the P_BAD for the synthesis of the enzyme needed for the catabolism of L-Arabinose. PBAD contains three half sites, each of which binds to a subunit of ARAC-O2, I1, and I2. The O1 site is composed of O1L and O1R half sites that bind two subunits. Half of O2 is in the araC coding region. We connected the target genes downstream of P_BAD to constitute the effect part of arabinose sensing system, and constructed the whole arabinose sensing system on the same plasmid with P_C and araC.
Arabinose induces endotoxin expression: (链接a01+c07)
Arabinose induces green fluorescent protein expression: (链接a01+c09)

Sequence and Features