Difference between revisions of "Part:BBa K3402057"
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<partinfo>BBa_K3402057 short</partinfo>
 
<partinfo>BBa_K3402057 short</partinfo>
  
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This device is composed of 50bp-up<i>PXA1</i>(BBa_K3402037), <i>hph</i>(BBa_K3402012), 50bp-do<i>PXA1</i>(BBa_K3402038), P<i>tef1</i>(BBa_K3402007), <i>RS</i> and <i>HH</i>(BBa_K3402029), sg<i>PXA1</i>(BBa_K3402039), <i>HDV</i>(BBa_K3402028), <i>RS</i> and <i>HH</i>(BBa_K3402029), sg<i>GFP</i>(BBa_K3402024), <i>HDV</i>(BBa_K3402028), T<i>syn7</i>(BBa_K3402001), up<i>GFP</i>(BBa_K3402025), do<i>GFP</i>(BBa_K3402026).
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This device is composed of 50bp-up<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402037# BBa_K3402037]), <i>hph</i>([https://parts.igem.org/Part:BBa_K3402012# BBa_K3402012]), 50bp-do<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402038# BBa_K3402038]), P<i>tef1</i>([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), <i>RS</i> and <i>HH</i>([https://parts.igem.org/Part:BBa_K3402029# BBa_K3402029]), sg<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402039# BBa_K3402039]), <i>HDV</i>([https://parts.igem.org/Part:BBa_K3402028# BBa_K3402028]), <i>RS</i> and <i>HH</i>([https://parts.igem.org/Part:BBa_K3402029# BBa_K3402029]), sg<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402024# BBa_K3402024]), <i>HDV</i>([https://parts.igem.org/Part:BBa_K3402028# BBa_K3402028]), T<i>syn7</i>([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]), up<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402025# BBa_K3402025]), do<i>GFP</i>([https://parts.igem.org/Part:BBa_K3402026# BBa_K3402026]).
  
 
[[Image:.png|700px|center|]]
 
[[Image:.png|700px|center|]]
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We realize the knock-out of <i>PXA1</i> and <i>yeGFP</i> genes. After observing the strains that growed on the plate coated with hygromycin can also lose the function of expressing green fluorescence, we got the conclusion that the success of double gene editing was proved.
 
We realize the knock-out of <i>PXA1</i> and <i>yeGFP</i> genes. After observing the strains that growed on the plate coated with hygromycin can also lose the function of expressing green fluorescence, we got the conclusion that the success of double gene editing was proved.
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[[Image:.png|700px|thumb|center|Left: ]]
  
 
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Revision as of 10:39, 22 October 2020


Double-gene editing cassette

This device is composed of 50bp-upPXA1(BBa_K3402037), hph(BBa_K3402012), 50bp-doPXA1(BBa_K3402038), Ptef1(BBa_K3402007), RS and HH(BBa_K3402029), sgPXA1(BBa_K3402039), HDV(BBa_K3402028), RS and HH(BBa_K3402029), sgGFP(BBa_K3402024), HDV(BBa_K3402028), Tsyn7(BBa_K3402001), upGFP(BBa_K3402025), doGFP(BBa_K3402026).

File:.png

Usage and Biology

We realize the knock-out of PXA1 and yeGFP genes. After observing the strains that growed on the plate coated with hygromycin can also lose the function of expressing green fluorescence, we got the conclusion that the success of double gene editing was proved.

File:.png
Left:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2807