Difference between revisions of "Part:BBa K3402055"

Revision as of 10:13, 22 October 2020

Over-expression UGTB cassette

This device is composed of 50bp-upPXA1(BBa_K3402037), Six site(BBa_K3402032), Pgalk(BBa_K3402033), Rec(BBa_K3402034), Tgki(BBa_K3402019), hph(BBa_K3402012), Ptef1(BBa_K3402007), UGTB(BBa_K3402011), Tsyn7(BBa_K3402001), 50bp-doPXA1(BBa_K3402038).

File:.png

Usage and Biology

upPXA1 and doPXA1 are homologous arms, which means this device will edit the PXA1 site. The β-Rec/six self-excising system will help to recycle the hygromycin resistance gene. UGTB is the key gene in the synthesis of sophorolipid.
When we add this fragment to the Cas9 expression device, we will use promoters with different expression strength as we characterized before to control the expression level of UGTB. Then we can produce different amount of lactone-type sophorolipids.
When the over-expression of SBLE and UGTB both work in the cell, there will be two type of sophorolipids produced by our engineering yeast, acid type and lactone type. Different types of sophorolipids have different functions. The combination of lactone type and acid type may increase the effect of sophorolipid. So, we can control the ratio of lactone and acid type sophorolipids.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1076
Illegal BglII site found at 5893
Illegal BamHI site found at 3479
Illegal XhoI site found at 1910
Illegal XhoI site found at 4631
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 219
Illegal AgeI site found at 5315
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 5474
Illegal BsaI.rc site found at 5429

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3402055 short</partinfo>
-This device is composed of 50bp-up<i>PXA1</i>(BBa_K3402037), Six site(BBa_K3402032), P<i>galk</i>(BBa_K3402033), <i>Rec</i>(BBa_K3402034), T<i>gki</i>(BBa_K3402019), <i>hph</i>(BBa_K3402012), P<i>tef1</i>(BBa_K3402007), <i>UGTB</i>(BBa_K3402011), T<i>syn7</i>(BBa_K3402001), 50bp-do<i>PXA1</i>(BBa_K3402038).
+This device is composed of 50bp-up<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402037# BBa_K3402037]), Six site([https://parts.igem.org/Part:BBa_K3402032# BBa_K3402032]), P<i>galk</i>([https://parts.igem.org/Part:BBa_K3402033# BBa_K3402033]), <i>Rec</i>([https://parts.igem.org/Part:BBa_K3402034# BBa_K3402034]), T<i>gki</i>([https://parts.igem.org/Part:BBa_K3402019# BBa_K3402019]), <i>hph</i>([https://parts.igem.org/Part:BBa_K3402012# BBa_K3402012]), P<i>tef1</i>([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), <i>UGTB</i>([https://parts.igem.org/Part:BBa_K3402011# BBa_K3402011]), T<i>syn7</i>([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]), 50bp-do<i>PXA1</i>([https://parts.igem.org/Part:BBa_K3402038# BBa_K3402038]).
+[[Image:.png|700px|center|]]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+up<i>PXA1</i> and do<i>PXA1</i> are homologous arms, which means this device will edit the <i>PXA1</i> site. The β-Rec/six self-excising system will help to recycle the hygromycin resistance gene. <i>UGTB</i> is the key gene in the synthesis of sophorolipid.
+<br>
+When we add this fragment to the Cas9 expression device, we will use promoters with different expression strength as we characterized before to control the expression level of <i>UGTB</i>. Then we can produce different amount of lactone-type sophorolipids.
+<br>
+When the over-expression of <i>SBLE</i> and <i>UGTB</i> both work in the cell, there will be two type of sophorolipids produced by our engineering yeast, acid type and lactone type. Different types of sophorolipids have different functions. The combination of lactone type and acid type may increase the effect of sophorolipid. So, we can control the ratio of lactone and acid type sophorolipids.
 <!-- -->