Difference between revisions of "Part:BBa K136064"

Revision as of 02:10, 29 October 2008

fliL promoter + WT RBS ( K136009) - EnvZ*

EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR.

fliL is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ).

Its promoter is consitutively repressed.

It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

This construction may allow ,with the add of a reporter tag, to see some oscillations

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 476
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 476
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 476
Illegal BglII site found at 396
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 476
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 476
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K136064 short</partinfo>
@@ Line 11: / Line 10: @@
 It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
-This construction may allow ,with the add of a reporter tag, to see some oscillations
+<br>This construction may allow ,with the add of a reporter tag, to see some oscillations
 <!-- Add more about the biology of this part here