Difference between revisions of "Part:BBa K3447007"

Revision as of 08:47, 22 October 2020

xynD, which produces xylanase

P_BAD promoter is an inducible promoter, P_BAD contains sequences of PC. It was verified by iGEM 2017 Amazonas_Brazil team that the induction effect of arabinose could be realized by using only araC and P_BAD.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 216
Illegal PstI site found at 226
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 216
Illegal PstI site found at 226
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 216
Illegal PstI site found at 226
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 216
Illegal PstI site found at 226
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3447007 short</partinfo>
-P<sub>C</sub> is a constitutive promoter that expresses the downstream <i>araC</i> gene.P<sub>BAD</sub> promoter is an inducible promoter, P<sub>BAD</sub> contains sequences of PC. It was verified by iGEM 2017 Amazonas_Brazil team that the induction effect of arabinose could be realized by using only <i>araC</i> and P<sub>BAD</sub>.
+P<sub>BAD</sub> promoter is an inducible promoter, P<sub>BAD</sub> contains sequences of PC. It was verified by iGEM 2017 Amazonas_Brazil team that the induction effect of arabinose could be realized by using only <i>araC</i> and P<sub>BAD</sub>.<br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+In the arabinose operon, it is regulated by the AraC operon and activates the expression of downstream arabinose metabolism genes in the presence of arabinose inducers.<br>
+The L-Arabinose operon is naturally present in <i>E. coli</i>.The regulatory protein AraC ACTS as a dimer. The operon contains two cis-type control elements: The P<sub>C</sub> promoter for the synthesis of AraC and the P<sub>BAD</sub> for the synthesis of the enzyme needed for the catabolism of L-Arabinose. P<sub>BAD</sub> contains three half sites, each of which binds to a subunit of ARAC-O2, I1, and I2. The O1 site is composed of O1L and O1R half sites that bind two subunits. Half of O2 is in the araC coding region.
+We connected the target genes downstream of P<sub>BAD</sub> to constitute the effect part of arabinose sensing system, and constructed the whole arabinose sensing system on the same plasmid with P<sub>C</sub> and araC.
+Arabinose induces endotoxin expression: <partinfo>BBa_K3447000</partinfo>
+Arabinose induces green fluorescent protein expression: (链接a01+c09)
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