Difference between revisions of "Part:BBa K3504011"

Revision as of 08:01, 22 October 2020

Multi-Epitope TNBC Vaccine Version (1)

Part Description

A multi-epitope vaccine formed of highly expressed and specific TNBC neo-epitopes and specifically chosen according to egyptian population alleles which can work as a generalized vaccine and also personalized vaccine which would illicit an immune response specific to TNBC tumor cells

Usage

An adjuvant (45 amino acid long β-defensin) was bound with the aid of the EAAAK linker at the start (to the MEV N-terminal). EAAAK linker reduces connection with other protein areas with efficient detachment and increases stability [88,89]. The vaccine’s immunogenicity may increase with an adjuvant. Epitopes were merged together based on their interaction’s compatibility in a sequential manner with AAY and GPGPG linkers, respectively. AAY and GPGPG prevent the production of junctional epitopes, that is the main task in the construction of multiepitope vaccines; on the other hand, they promote the immunization and epitope presentation.

Characterization

Improvements

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 120
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 120
Illegal NotI site found at 44
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 120
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 120
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 584
Illegal BsaI.rc site found at 935
Illegal SapI.rc site found at 853

@@ Line 6: / Line 6: @@
 A multi-epitope vaccine formed of highly expressed and specific TNBC neo-epitopes and specifically chosen according to egyptian population alleles which can work as a generalized vaccine and also personalized vaccine which would illicit an immune response specific to TNBC tumor cells
 ==Usage==
+An adjuvant (45 amino acid long β-defensin) was bound with the aid of the EAAAK linker at the start (to the MEV N-terminal). EAAAK linker reduces connection with other protein areas with efficient detachment and increases stability [88,89]. The vaccine’s immunogenicity may increase with an adjuvant. Epitopes were merged together based on their interaction’s compatibility in a sequential manner with AAY and GPGPG linkers, respectively. AAY and GPGPG prevent the production of junctional epitopes, that is the main task in the construction of multiepitope vaccines; on the other hand, they promote the immunization and epitope presentation.
 ==Characterization==
 ==Improvements==