Difference between revisions of "Part:BBa K3408002"

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<partinfo>BBa_k3408002 short</partinfo>
 
<partinfo>BBa_k3408002 short</partinfo>
  
 
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It is the part of toehold switch which functions as the key to relieve switch RNA and activate downstream circuit.  
We would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.  
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We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
 
We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
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 +
In our project we would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.
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https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
 
https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png

Revision as of 04:53, 22 October 2020

Trigger RNA(of toehold)

It is the part of toehold switch which functions as the key to relieve switch RNA and activate downstream circuit.

We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.

In our project we would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.


T--NAU-CHINA--basic-parts2.png Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]