Difference between revisions of "Part:BBa K3408002"
NJAU-Chappie (Talk | contribs) |
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<partinfo>BBa_k3408002 short</partinfo> | <partinfo>BBa_k3408002 short</partinfo> | ||
− | + | It is the part of toehold switch which functions as the key to relieve switch RNA and activate downstream circuit. | |
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We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability. | We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability. | ||
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+ | In our project we would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide. | ||
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https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png | https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png |
Revision as of 04:53, 22 October 2020
Trigger RNA(of toehold)
It is the part of toehold switch which functions as the key to relieve switch RNA and activate downstream circuit.
We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
In our project we would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.
Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 110
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]