Difference between revisions of "Part:BBa K3408000"

Revision as of 04:42, 22 October 2020

promoter nar(fnr regulated)

We add the promoter “P_nar”, in response to the RES-FNR oxygen regulator system of Bacillus Subtilis, to express Phy(ycD) efficiently in the absence of oxygen, and meanwhile express CI repressor protein to inhibit toehold switch-mzaF downstream.

According to the paper, they have designed three kinds of strength of the promoter P_nar and applied them in E.coli successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter.

Fig.1. The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized(Hwang Hee Jin, et al., BIOTECHNOL BIOFUELS. 2018)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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−	We add the promoter “P<sub>nar</sub>”, <b>in response to the RES-FNR oxygen regulator system</b> of <i>Bacillus Subtilis</i>, to express <b>~~phy~~(ycD) ~~phytase~~</b> efficiently in the absence of oxygen, and meanwhile express <b>CI repressor protein</b> to inhibit toehold switch-mzaF downstream.	+	We add the promoter “P<sub>nar</sub>”, <b>in response to the RES-FNR oxygen regulator system</b> of <i>Bacillus Subtilis</i>, to express <b>Phy(ycD)</b> efficiently in the absence of oxygen, and meanwhile express <b>CI repressor protein</b> to inhibit toehold switch-mzaF downstream.

	According to the paper, they have designed three kinds of strength of the promoter P<sub>nar</sub> and applied them in <i>E.coli</i> successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter.		According to the paper, they have designed three kinds of strength of the promoter P<sub>nar</sub> and applied them in <i>E.coli</i> successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter.