Difference between revisions of "Part:BBa K3408003"

Revision as of 04:37, 22 October 2020

Switch RNA(of toehold)

It is the part of toehold switch which functions as the controller of suicide switch. It is activated by P_CI when oxygen exists in wormcast.

We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the Switch RNA to mazF, following the P_CI downstream

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 8
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 It is the part of toehold switch which functions as the controller of suicide switch. It is activated by P<sub>CI</sub> when oxygen exists in wormcast.
-We hope our engineered bacteria not express Dpnc accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the Switch RNA to mazF, following the P<sub>CI</sub> downstream
+We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the Switch RNA to mazF, following the P<sub>CI</sub> downstream
 <!-- Add more about the biology of this part here
 ===Usage and Biology===