Difference between revisions of "Part:BBa K3408002"
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<span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span> | <span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 04:34, 22 October 2020
Trigger RNA(of toehold)
We would use the Trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.
We modifed our Trigger RNA with some bps in the 5’ terminal to increase its stability.
Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 110
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]