Difference between revisions of "Part:BBa K3328013"
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===Design=== | ===Design=== | ||
The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state. | The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state. | ||
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| + | https://2020.igem.org/wiki/images/thumb/1/1b/T--OUC-China--design_lunbo_and.jpg/799px-T--OUC-China--design_lunbo_and.jpg | ||
===Result=== | ===Result=== | ||
Revision as of 02:49, 22 October 2020
switch of XOR gate (XOR1)
realize the boolean calculation of XOR
Design
The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.
Result
The left figure shows that when INPUT A=1, INPUT B=0, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This didn't correspond to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 746