Difference between revisions of "Part:BBa K3506011"
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It is the promoter of GAL1 gene, which codes galactokinase. For our project，we use it to express GFP fused with the first 124 amino acid of Clb2，then release the induction，if the fluorescence disappears，it can be proved that the first 124 amino acid of Clb2 have the ability to help proteins degrade. | It is the promoter of GAL1 gene, which codes galactokinase. For our project，we use it to express GFP fused with the first 124 amino acid of Clb2，then release the induction，if the fluorescence disappears，it can be proved that the first 124 amino acid of Clb2 have the ability to help proteins degrade. | ||
| + | <font size="4"><b>Biology and Usage</b></font> | ||
It can be generally induced for gene expression by galactose and inhibited by glucose [1][2], making it a highly efficient inducible promoter for expression of foreign protein. | It can be generally induced for gene expression by galactose and inhibited by glucose [1][2], making it a highly efficient inducible promoter for expression of foreign protein. | ||
Revision as of 14:24, 21 October 2020
GAL1 promoter
It is the promoter of GAL1 gene, which codes galactokinase. For our project，we use it to express GFP fused with the first 124 amino acid of Clb2，then release the induction，if the fluorescence disappears，it can be proved that the first 124 amino acid of Clb2 have the ability to help proteins degrade.
Biology and Usage It can be generally induced for gene expression by galactose and inhibited by glucose [1][2], making it a highly efficient inducible promoter for expression of foreign protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 70
- 1000COMPATIBLE WITH RFC[1000]