Difference between revisions of "Part:BBa K3582026"

Revision as of 10:13, 21 October 2020

Inhibitory sequence 1 of PfEMP1 - ICAM1 interaction grafted into a cyclotide

This biobrick is able to synthesize the grafted cyclotide that could inhibit the 5MZA interaction between the host protein (ICAM-1) and the parasite protein (PfEMP1). The different components of this biobrick are described below:

1] BBa_K3582020: C intein, which is the modified version of the biobrick used by the 2014 iGEM Heidelberg team.
2] BBa_K2333015: Bsa 1 Reversed recognition site.
3] BBA_K3582023(The cyclotide precursor): The gene containing the cyclotide Kalata B1(29 amino acids), which is inserted between the C intein and the N intein so that it will circularize after translation.
4] Strep Ⅱ tag: This tag is placed within loop 3 of the cyclotide construct which could reduce the hemolytic activity of the cyclotide. This tag is also used to purify the molecule.
5] BBa_K3582006: Inhibitory sequence 1, which is thought to be able to inhibit the interaction between ICAM-1 and PfEMP1, is grafted into loop 6 of the cyclotide. This inhibitor has a higher affinity for interaction as compared to the wild type.
6] BBa_K3582021: Bsa 1 recognition site
7] BBa_K3582022: N intein, which is the modified version of the biobrick used by the Heidelberg 2014 team for circularization of the protein.

This final product is thought to be able to act like a drug that is capable of preventing the ICAM-1 and PfEMP1 interaction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 112
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 143
Illegal BsaI.rc site found at 109

@@ Line 6: / Line 6: @@
 This biobrick is able to synthesize the grafted cyclotide that could inhibit the 5MZA interaction between the host protein (ICAM-1) and the parasite protein (PfEMP1). The different components of this biobrick are described below:
-] BBa_k1362401: C intein, which is the modified version of the biobrick used by the 2014 iGEM Heidelberg team.
+*1] BBa_K3582020: C intein, which is the modified version of the biobrick used by the 2014 iGEM Heidelberg team.
-] BBa_k1362400: N intein, which is the modified version of the biobrick used by the 2014 iGEM Heidelberg team.
+*2] BBa_K2333015: Bsa 1 Reversed recognition site.
-] The cyclotide precursor: The gene containing the cyclotide Kalata B1(29 amino acids), which is inserted between the C intein and the N intein so that it will circularize after translation.
+*3] BBA_K3582023(The cyclotide precursor): The gene containing the cyclotide Kalata B1(29 amino acids), which is inserted between the C intein and the N intein so that it will circularize after translation.
-] Strep &#8545; tag: This tag is placed within loop 3 of the cyclotide construct which could reduce the hemolytic activity of the cyclotide. This tag is also used to purify the molecule.
+*4] Strep &#8545; tag: This tag is placed within loop 3 of the cyclotide construct which could reduce the hemolytic activity of the cyclotide. This tag is also used to purify the molecule.
-] BBa_kK3582006 : Inhibitory sequence 1, which is thought to be able to inhibit the interaction between ICAM-1 and PfEMP1, is grafted into loop 6 of the cyclotide. This inhibitor has a higher affinity for interaction as compared to the wild type.
+*5] BBa_K3582006: Inhibitory sequence 1, which is thought to be able to inhibit the interaction between ICAM-1 and PfEMP1, is grafted into loop 6 of the cyclotide. This inhibitor has a higher affinity for interaction as compared to the wild type.
+*6] BBa_K3582021: Bsa 1 recognition site
+*7] BBa_K3582022: N intein, which is the modified version of the biobrick used by the Heidelberg 2014 team for circularization of the protein.
 This final product is thought to be able to act like a drug that is capable of preventing the ICAM-1 and PfEMP1 interaction.