Difference between revisions of "Part:BBa K3510000"

Revision as of 08:03, 21 October 2020

FAST2 reporter gene

This part encodes for the Fluorescence-Activating and Absorption-Shifting Tag (FAST) protein, a 14 kDAsmall protein, that assembles with various fluorgenic ligands (Fluorogens) [1]. Only upon assembly a fluorescent signal is emitted and allows for detection and imaging with high contrast [1].

The original sequence was obtained from the Twinkle Factory and edited in one base pair to eliminate a PstI binding site, so it now follows iGEM’s assembly rules. In our project it was used as a fluorescent tag of the following DNA fragment, either the phytochelatin or chromoprotein.

Sequence and Features

References

1. The Twinkle Factory. The science behind FAST and SplitFAST [cited 2020 Oct 19]. Available from: URL: https://www.the-twinkle-factory.com/the-science-behind-fast-and-splitfast.