Difference between revisions of "Part:BBa K3510005"

 
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This composite part is a manganese inducible expression system of a phytochelatin (BBa_K1321005) tagged with FAST2 (BBa_K3510000). Expression is controlled by two individual elements: A strong Anderson promoter (BBa_J23102) and a manganese riboswitch (BBa_K902074). With this promoter choice transcription will constitutively happen, but translation initiation is dependent on manganese binding to the riboswitch. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015). This construct allows to determine the efficiency of the riboswitch by itself, without the support of the manganese promoter.
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In our project we coupled this manganese sensing device with a phytochelatin in order to create a bifunctional biosensor, that is not only able to detect manganese, but also directly binds it. With our suggested implementation this would contribute to the increase of water quality. Unlike the biosensor with the chromoprotein, the FAST2 tag allows a quantitative measurement of the fluorescence, which should provide information about the manganese concentration in the sample.
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===Sequence and Features===
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<partinfo>BBa_K3510005 SequenceAndFeatures</partinfo>
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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Revision as of 07:45, 21 October 2020

Anderson-Promoter-Mn-Riboswitch-FAST-Phytochelatine-Terminator

This composite part is a manganese inducible expression system of a phytochelatin (BBa_K1321005) tagged with FAST2 (BBa_K3510000). Expression is controlled by two individual elements: A strong Anderson promoter (BBa_J23102) and a manganese riboswitch (BBa_K902074). With this promoter choice transcription will constitutively happen, but translation initiation is dependent on manganese binding to the riboswitch. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015). This construct allows to determine the efficiency of the riboswitch by itself, without the support of the manganese promoter.

In our project we coupled this manganese sensing device with a phytochelatin in order to create a bifunctional biosensor, that is not only able to detect manganese, but also directly binds it. With our suggested implementation this would contribute to the increase of water quality. Unlike the biosensor with the chromoprotein, the FAST2 tag allows a quantitative measurement of the fluorescence, which should provide information about the manganese concentration in the sample.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 147