Difference between revisions of "Part:BBa K3328032"

 
m
Line 3: Line 3:
 
<partinfo>BBa_K3328032 short</partinfo>
 
<partinfo>BBa_K3328032 short</partinfo>
  
−
1
 
  
−
<!-- Add more about the biology of this part here
+
realize the boolean calculation of XOR
−
===Usage and Biology===
+
===Design===
 +
The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.
 +
 
 +
===Result===
 +
https://2020.igem.org/wiki/images/thumb/7/70/T--OUC-China--part_XOR1.jpg/800px-T--OUC-China--part_XOR1.jpg
 +
 
 +
The left figure shows that when INPUT A=1, INPUT B=0, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This didn't correspond to the situation described in the truth table on the right.  INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
 +
 
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K3328032 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K3328013 SequenceAndFeatures</partinfo>
 +
 
  
  

Revision as of 17:46, 20 October 2020


switch of XOR gate (XOR1)


realize the boolean calculation of XOR

Design

The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.

Result

800px-T--OUC-China--part_XOR1.jpg

The left figure shows that when INPUT A=1, INPUT B=0, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This didn't correspond to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 746