Difference between revisions of "Part:BBa K3328027"

Revision as of 17:30, 20 October 2020

switch of OR gate

realize the boolean calculation of OR

Design

OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation.

Result

The left figure shows that when INPUT A=0, INPUT B=0, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 819

@@ Line 4: / Line 4: @@
 realize the boolean calculation of OR
+===Design===
+OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation.
+===Result===
+https://2020.igem.org/wiki/images/thumb/6/6c/T--OUC-China--part_OR.jpg/800px-T--OUC-China--part_OR.jpg
+The left figure shows that when INPUT A=0, INPUT B=0, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
 <!-- Add more about the biology of this part here