Difference between revisions of "Part:BBa K2904111"
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==<strong>2020 OUC-China</strong>== | ==<strong>2020 OUC-China</strong>== | ||
| + | ===Design=== | ||
It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates. | It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates. | ||
Revision as of 17:27, 20 October 2020
aTc inducible Tet promoter
This promoter is induced by aTc. It was used by 2019 OUC-China team.
2020 OUC-China
Design
It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates.
Result
The Tet promoter fluorescence data in BL21 (DE3) at different aTc concentrations (0mg/ml, 0.01mg/ml, 0.1mg/ml, 0.25mg/ml, 0.5mg/ml and 1mg/ml). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/ml.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]