Difference between revisions of "Part:BBa K136046:Design"

(→‎References)
(→‎Source)
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===Source===
 
===Source===
  
−
bacterial genome of mutated (I281S) strain for EnvZ.
+
bacterial genome of mutated (I281S) MG1655 strain for EnvZ.
 +
 
 +
*Dissecting the Genetic Components of Adaptation of ''Escherichia coli'' to the Mouse Gut
 +
Giraud A, Arous S, Gaboriau-Routhiau V, De Paepe M, Bambou JC, et al. PLoS Genetics January 2008
  
 
===References===
 
===References===

Revision as of 23:23, 28 October 2008

envZ*


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR and ligation in pCR4Blunt-TOPO


Source

bacterial genome of mutated (I281S) MG1655 strain for EnvZ.

  • Dissecting the Genetic Components of Adaptation of Escherichia coli to the Mouse Gut

Giraud A, Arous S, Gaboriau-Routhiau V, De Paepe M, Bambou JC, et al. PLoS Genetics January 2008

References

EnvZ-OmpR Interaction and Osmoregulation in Escherichia coli,
Sheng Jian Cai and Masayori Inouye (2002),
THE JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 277, No. 27