Difference between revisions of "Part:BBa K3328008"
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an ON-switch to regulate the expression of the downstream gene | an ON-switch to regulate the expression of the downstream gene | ||
| + | ===Design=== | ||
| + | Toehold switch is composed of cis-acting element RNA hairpins and trans-acting factor trigger RNA. The binding of a trigger RNA to the toehold sequence allows for a branch migration process, exposing AUG and RBS for translation initiation. | ||
| + | ===Result=== | ||
| + | <html> | ||
| + | <head> | ||
| + | <meta charset="utf-8"> | ||
| + | |||
| + | </head> | ||
| + | <body> | ||
| + | <div> | ||
| + | <img src="https://2020.igem.org/wiki/images/7/7e/T--OUC-China--result_fig2_th.jpg" style="width: 80%;"> | ||
| + | </div> | ||
| + | |||
| + | <p> | ||
| + | Compared with the blank control (IPTG=0 M, aTc=0 mg/ml), the fluorescence of GFP was low when only the promoter before toehold sequence was turned on (IPTG=0.1 M, aTc=0 mg/ml). This indicates that the toehold has the advantage of low leakage. When the trigger was expressed (IPTG=0.1 M, aTc=0.25 mg/ml), it showed a high GFP fluorescence of up to 32.3-fold due to the destruction of toehold hairpin structure. | ||
| + | </p> | ||
| + | </body> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | ===User Reviews=== | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 16:48, 20 October 2020
ON-switch
an ON-switch to regulate the expression of the downstream gene
Design
Toehold switch is composed of cis-acting element RNA hairpins and trans-acting factor trigger RNA. The binding of a trigger RNA to the toehold sequence allows for a branch migration process, exposing AUG and RBS for translation initiation.
Result
Compared with the blank control (IPTG=0 M, aTc=0 mg/ml), the fluorescence of GFP was low when only the promoter before toehold sequence was turned on (IPTG=0.1 M, aTc=0 mg/ml). This indicates that the toehold has the advantage of low leakage. When the trigger was expressed (IPTG=0.1 M, aTc=0.25 mg/ml), it showed a high GFP fluorescence of up to 32.3-fold due to the destruction of toehold hairpin structure.
User Reviews
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]